P-185: Hawthorn Berries Ethanolic Extract Lowered Polycystic Ovarian Syndrome- Elevated RNA Damage in Follicular Cells

Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder. Various constituents including flavonoids with biological activities including free radical scavenging, anti-lipoperoxidation and anti-inflammatory have been isolated from hawthorn. Due to adverse effect of PCOS on ovarian vascular remodeling as well as PCOS-induced oxidative stress, we aimed to study the hawthorn’s berries hydroalcoholic extract potential effects on estradiol valerate-induced PCOS in rats. Materials and Methods: PCOS was induced in 3 groups of rats (n = 6) by intramuscular injection of estradiol valerate (EV, 4 mg/ratin 0.2 ml sesame oil). The control group only received the vehicle (0.2 ml sesame oil). A 60-day interval was chosen to let the cysts become established. Following the interval time, effects of hawthorn berries extract (HBE, 100 mg/kg, orally) and oral contraceptive pills (OCP, containing 60 μg/kg estradiol, orally) on PCO-induced RNA damage and Angiogenesis were evaluated. The RNA damage and angiogenesis were evaluated by using Bicki’s method and CD31+ immuno-fluorescent analyses, respectively. Results: Observations demonstrated that HBE significantly (p<0.05) reduced PCO-induced RNA damage and enhanced the micro and macro vessels distribution per one mm2 of the ovarian tissue. Conclusion: Our data suggest that HBE partly reduce the PCOS-induced damages on the ovary by up-regulating the angiogenesis process and down-regulating the RNA damage.